In vivo pathogenesis study of Flavobacterium psychrophilum infection on rainbow trout (Oncorhynchus mykiss) and its potential treatment with essential oils (EO).

Student: 
Isabella villaquiran

This thesis explores alternative approaches to managing Flavobacterium psychrophilum
infections in rainbow trout, a key challenge in salmonid aquaculture. In response to rising
antibiotic resistance and limited vaccine options, cinnamon and thyme essential oils (CEO and
TEO) were evaluated for their antimicrobial activity using MIC assays.
A preliminary immersion challenge using 10⁷ CFU/mL was conducted to validate the dose and
observe fish responses. The final trial combined the immersion challenge using 108 CFU/mL
with the essential oil treatments to assess effects on clinical signs and bacterial load.
Mortality was minimal, and only one case was recorded. Re-isolation of bacteria was
performed, but qPCR confirmation of the administered strains was unsuccessful. Samples and
isolates have been preserved for future analysis.
These findings suggest that essential oils may be promising candidates for further study in
disease management strategies for aquaculture. However, limitations in infection consistency
and strain confirmation underline the need for continued research.

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