Invasive alien species (IAS) pose ecological threats yet offer underexplored potential as sources of cosmeceutical bioactives. This study explores the biotechnological value of Acacia saligna, Carpobrotus edulis (halophytes), Rugulopteryx okamurae (brown macroalga), and their mix. Extraction using ethanol and water preceded the in vitro antioxidant (DPPH, ABTS, FRAP), enzyme inhibition (tyrosinase, elastase, lipase), and cytotoxicity assays on tumoural and non-tumoural mammalian cell lines. Untargeted metabolomic profiling via LC-HRMS/MS annotated over 100 putative bioactive compounds, including flavonoids and lipids. Overall, ethanol extracts showed higher bioactivity than water extracts. A. saligna extract exhibited potent antioxidant (ABTS EC₅₀ = 123 µg/mL; FRAP EC₅₀ = 78 µg/mL) and anti-tyrosinase (IC₅₀ = 160 µg/mL) activities. C. edulis extract exhibited strong DPPH scavenging (EC₅₀ = 14.8 µg/mL), while R. okamurae extract showed strong elastase inhibition (IC₅₀ = 32 µg/mL). Mixtures displayed synergistic, additive or antagonistic effects. Cytotoxicity assays confirmed the safety of A. saligna and Mix extracts in non-tumoural cell lines, while R. okamurae’s non-selective cytotoxicity was mitigated when combined with A. saligna. Prototype gels and lotions with A. saligna extract influenced pH (>6.0), higher than dermocosmetic ideals (4.5 - 6.0). A business plan was developed, supporting the commercial viability of IAS-formulated products for cosmeceutical applications within circular bioeconomy frameworks.
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