The fish epidermis plays a vital role as a sensory medium, osmotic barrier, and protection against environmental factors and marine pathogens. However, its specific effects on fish skin are still poorly understood. To bridge this knowledge gap, we assessed a well-established two-chamber skin explant model's suitability for extended studies and its role in bacterial-induced skin ulcer development. Initial 24-hour experiments demonstrated similarities between the model and control skin, with minimal histological changes. However, during 48-hour exposures, significant differences emerged, revealing advanced exfoliation and apoptosis in the latter group. Hence, further refinements to the protocol and experimental design are crucial for establishing a robust foundation for prolonged investigations.
Moreover, we conducted groundbreaking bacterial infection experiments, using Vibrio strains (CECT 4600, 2BC) linked to ulceration. These studies provided valuable insights into the model's advantages and its applicability for exploring mechanisms behind bacterial skin infections. Overall, the two-chamber model presents a promising alternative for studying skin diseases in marine species, despite limitations observed in the rainbow trout experiment. By continually developing the model, we can better understand disease etiology and enhance health management in aquaculture settings.
Keywords: common dab (Limanda limanda), skin ulceration, ex-vivo, Vibrio tapetis
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