Student:
Bianca Bertotti
Through this thesis I have analysed the expression profiles of eight genes involved in neuromodulation and neurotransmission in the cephalopod mollusc Octopus vulgaris. To carry out the analysis, nine tissues belonging to five animals were considered to determine the expression profile of eight genes involved in neuromodulation and neurotransmission pathways. The genes were first identified through in-silico analysis followed by in-vivo analysis carried out through Real Time PCR. Immunohistochemistry was also performed to identify substance-P in the supraesophageal mass. Although the presence of some technical issues, I was able to identify some new insights regarding the molecular fingerprint of O. vulgaris neuronal pattern.
